A Field Trial of TCE Phytoremediation by Genetically Modified Poplars Expressing Cytochrome P450 2E1.
Identifieur interne : 001555 ( Main/Exploration ); précédent : 001554; suivant : 001556A Field Trial of TCE Phytoremediation by Genetically Modified Poplars Expressing Cytochrome P450 2E1.
Auteurs : Emily K. Legault [États-Unis] ; C Andrew James [États-Unis] ; Keith Stewart [États-Unis] ; Indulis Muiznieks [États-Unis] ; Sharon L. Doty [États-Unis] ; Stuart E. Strand [États-Unis]Source :
- Environmental science & technology [ 1520-5851 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Populus.
- métabolisme : Cytochrome P-450 CYP2E1.
- Arbres, Dépollution biologique de l'environnement, Trichloroéthylène.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Cytochrome P-450 CYP2E1.
- enzymology : Populus.
- Biodegradation, Environmental, Trees, Trichloroethylene.
Abstract
A controlled field study was performed to evaluate the effectiveness of transgenic poplars for phytoremediation. Three hydraulically contained test beds were planted with 12 transgenic poplars, 12 wild type (WT) poplars, or left unplanted, and dosed with equivalent concentrations of trichloroethylene (TCE). Removal of TCE was enhanced in the transgenic tree bed, but not to the extent of the enhanced removal observed in laboratory studies. Total chlorinated ethene removal was 87% in the CYP2E1 bed, 85% in the WT bed, and 34% in the unplanted bed in 2012. Evapotranspiration of TCE from transgenic leaves was reduced by 80% and diffusion of TCE from transgenic stems was reduced by 90% compared to WT. Cis-dichloroethene and vinyl chloride levels were reduced in the transgenic tree bed. Chloride ion accumulated in the planted beds corresponding to the TCE loss, suggesting that contaminant dehalogenation was the primary loss fate.
DOI: 10.1021/acs.est.5b04758
PubMed: 28463483
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Three hydraulically contained test beds were planted with 12 transgenic poplars, 12 wild type (WT) poplars, or left unplanted, and dosed with equivalent concentrations of trichloroethylene (TCE). Removal of TCE was enhanced in the transgenic tree bed, but not to the extent of the enhanced removal observed in laboratory studies. Total chlorinated ethene removal was 87% in the CYP2E1 bed, 85% in the WT bed, and 34% in the unplanted bed in 2012. Evapotranspiration of TCE from transgenic leaves was reduced by 80% and diffusion of TCE from transgenic stems was reduced by 90% compared to WT. Cis-dichloroethene and vinyl chloride levels were reduced in the transgenic tree bed. Chloride ion accumulated in the planted beds corresponding to the TCE loss, suggesting that contaminant dehalogenation was the primary loss fate.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">28463483</PMID><DateCompleted><Year>2017</Year><Month>12</Month><Day>11</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-5851</ISSN><JournalIssue CitedMedium="Internet"><Volume>51</Volume><Issue>11</Issue><PubDate><Year>2017</Year><Month>Jun</Month><Day>06</Day></PubDate></JournalIssue><Title>Environmental science & technology</Title><ISOAbbreviation>Environ Sci Technol</ISOAbbreviation></Journal><ArticleTitle>A Field Trial of TCE Phytoremediation by Genetically Modified Poplars Expressing Cytochrome P450 2E1.</ArticleTitle><Pagination><MedlinePgn>6090-6099</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.est.5b04758</ELocationID><Abstract><AbstractText>A controlled field study was performed to evaluate the effectiveness of transgenic poplars for phytoremediation. Three hydraulically contained test beds were planted with 12 transgenic poplars, 12 wild type (WT) poplars, or left unplanted, and dosed with equivalent concentrations of trichloroethylene (TCE). Removal of TCE was enhanced in the transgenic tree bed, but not to the extent of the enhanced removal observed in laboratory studies. Total chlorinated ethene removal was 87% in the CYP2E1 bed, 85% in the WT bed, and 34% in the unplanted bed in 2012. Evapotranspiration of TCE from transgenic leaves was reduced by 80% and diffusion of TCE from transgenic stems was reduced by 90% compared to WT. Cis-dichloroethene and vinyl chloride levels were reduced in the transgenic tree bed. Chloride ion accumulated in the planted beds corresponding to the TCE loss, suggesting that contaminant dehalogenation was the primary loss fate.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Legault</LastName><ForeName>Emily K</ForeName><Initials>EK</Initials><AffiliationInfo><Affiliation>Department of Civil and Environmental Engineering, UW Box 355014, University of Washington , Seattle, Washington, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>James</LastName><ForeName>C Andrew</ForeName><Initials>CA</Initials><AffiliationInfo><Affiliation>Center for Urban Waters, University of Washington Tacoma , Tacoma, Washington, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stewart</LastName><ForeName>Keith</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Civil and Environmental Engineering, UW Box 355014, University of Washington , Seattle, Washington, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Muiznieks</LastName><ForeName>Indulis</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>College of the Environment, School of Environmental and Forest Sciences, UW Box 352100, University of Washington , Seattle, Washington, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Doty</LastName><ForeName>Sharon L</ForeName><Initials>SL</Initials><AffiliationInfo><Affiliation>College of the Environment, School of Environmental and Forest Sciences, UW Box 352100, University of Washington , Seattle, Washington, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Strand</LastName><ForeName>Stuart E</ForeName><Initials>SE</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-6700-3498</Identifier><AffiliationInfo><Affiliation>Department of Civil and Environmental Engineering, UW Box 355014, University of Washington , Seattle, Washington, United States.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>05</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Environ Sci Technol</MedlineTA><NlmUniqueID>0213155</NlmUniqueID><ISSNLinking>0013-936X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>290YE8AR51</RegistryNumber><NameOfSubstance UI="D014241">Trichloroethylene</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.13.-</RegistryNumber><NameOfSubstance UI="D019392">Cytochrome P-450 CYP2E1</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="Y">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019392" MajorTopicYN="N">Cytochrome P-450 CYP2E1</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014241" MajorTopicYN="Y">Trichloroethylene</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>5</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>12</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>5</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28463483</ArticleId><ArticleId IdType="doi">10.1021/acs.est.5b04758</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Washington (État)</li></region></list><tree><country name="États-Unis"><region name="Washington (État)"><name sortKey="Legault, Emily K" sort="Legault, Emily K" uniqKey="Legault E" first="Emily K" last="Legault">Emily K. Legault</name></region><name sortKey="Doty, Sharon L" sort="Doty, Sharon L" uniqKey="Doty S" first="Sharon L" last="Doty">Sharon L. Doty</name><name sortKey="James, C Andrew" sort="James, C Andrew" uniqKey="James C" first="C Andrew" last="James">C Andrew James</name><name sortKey="Muiznieks, Indulis" sort="Muiznieks, Indulis" uniqKey="Muiznieks I" first="Indulis" last="Muiznieks">Indulis Muiznieks</name><name sortKey="Stewart, Keith" sort="Stewart, Keith" uniqKey="Stewart K" first="Keith" last="Stewart">Keith Stewart</name><name sortKey="Strand, Stuart E" sort="Strand, Stuart E" uniqKey="Strand S" first="Stuart E" last="Strand">Stuart E. Strand</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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